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Faculty and Staff

Kristi Koelsch, PhD
Internal Medicine

Kristi Koelsch, PhD

Associate Professor of Research


OMRF
825 NE 13th St. - MS 24
Oklahoma City, OK 73104

(405) 271-7797

Kristi-Koelsch@ouhsc.edu


Academic Section(s):

Endocrinology and Diabetes


Education:

  • 2009     Doctor of Philosophy, Focus in Immunogenetics, Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK
  • 2002     Bachelor of Science, University of Oklahoma, Norman, OK


Fellowship:

  • 2009 - 2013     Post-doctoral Fellow, Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK


Clinical/Research Interests:

Research Interests

My research interests are in autoimmune diseases, specifically B and T cells and the roles they play in the development of autoimmunity. My early research training included the characterization of B cell subset populations and the production of human monoclonal antibodies from various B cell subsets.  This led to the identification of two rare sub-populations of memory B cells that are found in all healthy humans, one that has class-switched to IgD (Cδ-CS) and expresses autoreactive antibodies and another that is naïve, autoreactive and anergic (BND).

My current projects include the characterization of B cells from Sjögren’s syndrome (SS) patients, which includes monoclonal antibody production from single-cell-sorted plasmablasts isolated from the salivary glands, antigen specificity and functional testing, Fab-region N-glycosylation analyses, as well as genetic repertoire analysis. The data we are able to obtain from this very rare population of infiltrating cells has allowed us to demonstrate that many of the autoreactive, infiltrating cells we have sorted from the glands are not only of the IgG isotype, but also of the IgA isotype. We have also reported increased immunoglobulin Fab-N-glycans in SS patients, and found evidence for alternate modes of B cell activation in the B cells that harbor Fab-N-glycans, which may be central to the increased risk for development of lymphoma in SS.

We are also evaluating early salivary biomarkers in non-SS sicca (chronic dry mouth, without SS) which may predict those most likely to progress to SS, as well as the immune cell subsets and microenvironments involved in the salivary gland ectopic germinal center formation occurring in SS, the mechanisms for production of autoantibodies, as well as studies to determine if the plasmablasts infiltrating the salivary glands of SS patients are indeed the source of the serum autoantibodies found in these patients.


Select Publications:

Scofield RH, Sharma R, Pezant N, Kelly JA, Radfar L, Lewis DM, Kaufman CE, Cioli S, Harris J, Grundahl K, Rhodus NL, Wallace DJ, Weisman MH, Venuturupalli S, Brennan MT, Koelsch KA, Lessard CJ, Montgomery CG, Sivils KL, Rasmussen A. American Indians Have A Higher Risk Of Sjögren's Syndrome And More Disease Activity Than Caucasians And African-Americans. Arthritis Care Res (Hoboken). 2019 Jun 14;. doi: 10.1002/acr.24003. [Epub ahead of print] PubMed PMID: 31199565.

Harris VM, Harley ITW, Kurien BT, Koelsch KA, Scofield RH. Lysosomal pH Is Regulated in a Sex Dependeent  Manner in Immune Ceells Expresssing CXorf21. Front Immunol. 2019;10:578. doi: 10.3389/fimmu.2019.00578. eCollection 2019. PubMed PMID: 31001245; PubMed Central PMCID: PMC6454867.

Scofield, R. H.#, Fayyaz, A., Kurien, B. T., Koelsch, K. A. (2018). Prognostic value of Sjögren’s syndrome autoantibodies. Journal of Laboratory and Precision Medicine. 2018 October; 3:92. doi: 10.21037/jlpm.2018.08.05.

Koelsch, K. A., Cavett, J., Smith, K. M., Moore, J. S., Lehoux, S. D., Jia, N., Mather, T., Quadri SMS, Rasmussen, A., Kaufman, C. E., Lewis, D. M., Radfar, L., Scordino, T. A., Lessard, C. J., Kurien, B. T., Cummings, R. D., James, J. A., Sivils, K. L., Darise Farris, A., Scofield, R. H. 2018). Evidence for Alternate Modes of B cell Activation Involving Fab Acquired-N-Glycosylations in Antibody Secreting Cells Infiltrating the Labial Salivary Glands of Sjögren's Syndrome Patients. Arthritis & Rheumatology (Hoboken, N.J.). PMID: 29457375. DOI: 10.1002/art.40458

Sharma, R., Harris, V. M., Cavett, J., Kurien, B. T., Liu, K., Koelsch, K. A., Sivils, K. L., Scofield, R. H. (2017). Rare X Chromosome Abnormalities in Systemic Lupus Erythematosus and Sjögren's Syndrome. Arthritis & Rheumatology (Hoboken, N.J.), 69(11), 2187-2192. PMID: 28692793. DOI: 10.1002/art.40207

Fedorowski, A., Li, H., Yu, X., Koelsch, K. A., Harris, V. M., Liles, C., Murphy, T. A., Quadri SMS, Scofield, R. H., Sutton, R., Melander, O., Kem, D. C. (2017). Antiadrenergic autoimmunity in postural tachycardia syndrome. Europace : European pacing, arrhythmias, and cardiac lectrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology, 19(7), 1211-1219. PMID: 27702852. DOI: 10.1093/europace/euw154

Harris, V. M., Sharma, R., Cavett, J., Kurien, B. T., Liu, K., Koelsch, K. A., Scofield, R. H. (2016). Klinefelter's syndrome (47,XXY) is in excess among men with Sjögren's syndrome. Clinical immunology (Orlando, Fla.), 168, 25-29. PMID: 27109640. DOI: 10.1016/j.clim.2016.04.002

Liu, K., Kurien, B. T., Zimmerman, S. L., Kaufman, K. M., Taft, D. H., Kottyan, L. C., Lazaro, S., Weaver, C. A., Ice, J. A., Adler, A. J., Chodosh, J., Radfar, L., Rasmussen, A., Stone, D. U., Lewis, D. M., Li, S., Koelsch, K. A., Harley, J. B., Scofield, R. H. (2016). X Chromosome Dose and Sex Bias in Autoimmune Diseases: Increased Prevalence of 47,XXX in Systemic Lupus Erythematosus and Sjögren's Syndrome. Arthritis & rheumatology (Hoboken, N.J.), 68(5), 1290-1300. PMID: 26713507. DOI: 10.1002/art.39560

Aggarwal, R., Anaya, J. M., Koelsch, K. A., Kurien, B. T., Scofield, R. H. (2015). Association between Secondary and Primary Sjögren's Syndrome in a Large Collection of Lupus Families. Autoimmune diseases, 2015, 298506. PMID: 26246904. DOI: 10.1155/2015/298506

Koelsch, K. A., Cavett, J., Smith, K. M., Moore, J. S., Lehoux, S. D., Jia, N., Mather, T., Quadri SMS, Rasmussen, A., Kaufman, C. E., Lewis, D. M., Radfar, L., Scordino, T. A., Lessard, C. J., Kurien, B. T., Cummings, R. D., James, J. A., Sivils, K. L., Darise Farris, A., Scofield, R. H. (2018). Evidence for Alternate Modes of B cell Activation Involving Fab Acquired-N-Glycosylations in Antibody Secreting Cells Infiltrating the Labial Salivary Glands of Sjögren's Syndrome Patients. Arthritis & Rheumatology (Hoboken, N.J.). PMID: 29457375. DOI: 10.1002/art.40458

Maier-Moore JS*, Koelsch KA*, Smith K, Lessard CJ, Radfar L, Lewis D, Kurien BT, Wolska N, Deshmukh U, Rasmussen A, Sivils KL, James JA, Farris AD, Scofield RH. Antibody-secreting cell specificity in labial salivary glands reflects the clinical presentation and serology in patients with Sjögren's syndrome. Arthritis Rheumatol. 2014 Dec;66(12):3445-56. PubMed PMID: 25199908; NIHMSID: NIHMS630529; PubMed Central PMCID: PMC4245382. *Authors contributed equally

Altorok N, Coit P, Hughes T, Koelsch KA, Stone DU, Rasmussen A, Radfar L, Scofield RH, Sivils KL, Farris AD, Sawalha AH. Genome-wide DNA methylation patterns in naive CD4+ T cells from patients with primary Sjögren's syndrome. Arthritis Rheumatol. 2014 Mar;66(3):731-9. PubMed PMID: 24574234; NIHMSID: NIHMS541595; PubMed Central PMCID: PMC4009982.

Dozmorov MG, Giles CB, Koelsch KA, Wren JD. Systematic classification of non-coding RNAs by epigenomic similarity. BMC Bioinformatics. 2013;14 Suppl 14:S2. PubMed PMID: 24267974; PubMed Central PMCID: PMC3851203.

Coit P, Jeffries M, Altorok N, Dozmorov MG, Koelsch KA, Wren JD, Merrill JT, McCune WJ, Sawalha AH. Genome-wide DNA methylation study suggests epigenetic accessibility and transcriptional poising of interferon-regulated genes in naïve CD4+ T cells from lupus patients. J Autoimmun. 2013 Jun;43:78- 84. PubMed PMID: 23623029; NIHMSID: NIHMS466777; PubMed Central PMCID: PMC3790645.

Koelsch KA, Wang Y, Maier-Moore JS, Sawalha AH, Wren JD. GFP affects human T cell activation and cytokine production following in vitro stimulation. PLoS One. 2013;8(4):e50068. PubMed PMID: 23577054; PubMed Central PMCID: PMC3618152.

Koelsch KA, Webb R, Jeffries M, Dozmorov MG, Frank MB, Guthridge JM, James JA, Wren JD, Sawalha AH. Functional characterization of the MECP2/IRAK1 lupus risk haplotype in human T cells and a human MECP2 transgenic mouse. J Autoimmun. 2013 Mar;41:168-74. PubMed PMID: 23428850; NIHMSID: NIHMS437189; PubMed Central PMCID: PMC3622940.

Duty JA, Szodoray P, Zheng NY, Koelsch KA, Zhang Q, Swiatkowski M, Mathias M, Garman L, Helms C, Nakken B, Smith K, Farris AD, Wilson PC. Functional anergy in a subpopulation of naive B cells from healthy humans that express autoreactive immunoglobulin receptors. J Exp Med. 2009 Jan 16;206(1):139-51. PubMed PMID: 19103878; PubMed Central PMCID: PMC2626668.

Koelsch K, Zheng NY, Zhang Q, Duty A, Helms C, Mathias MD, Jared M, Smith K, Capra JD, Wilson PC. Mature B cells class switched to IgD are autoreactive in healthy individuals. J Clin Invest. 2007 Jun;117(6):1558-65. PubMed PMID: 17510706; PubMed Central PMCID: PMC1866247.

Books & Chapters:

Koelsch, K. A. (in press). Single-Cell High Resolution Detection and Quantification of Protein Isoforms Differing by a Single Charge Unit. In Kurien, B. T., Scofield, R. H. (Eds.), Electrophoretic Separation of Proteins: Methods and Protocols. New York, NY: Springer Science+Business Media, LLC.

Shah, H. B., Koelsch, K. A. (2015). B-Cell ELISPOT: For the Identification of Antigen-Specific Antibody-Secreting Cells. (vol. 1312, pp. 419-26). Methods in molecular biology (Clifton, N.J.). PMID: 26044023. DOI: 10.1007/978-1-4939-2694-7_42

Leehan, K. M., Koelsch, K. A. (2015). T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells. (vol. 1312, pp. 427-34). Methods in molecular biology (Clifton, N.J.). PMID: 26044024. DOI: 10.1007/978-1-4939-2694-7_43