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Team Members

Dan Brobst, MS
Otolaryngology

Dan Brobst, MS

Sr. Staff Research Assistant


Dan received his Bachelors of Science in Biology from Baker University and Master's degree in Pharmacology from the University of Kansas. Dan has extensive experience in molecular biology, biochemistry, and pharmacology. He joined OUHSC in 2010 and Dr. Queimado's lab in 2016. His research interests involve examining health effects of electronic cigarettes, and specifically their role on DNA damage, innate immunity and inflammation.


Select Publications:

Recent Publications:

1. Brobst D, Manyanga J, Georgescu C, Wren J, Queimado L. Exposure to E-cigarette aerosol reduces the expression of Toll-like receptor 3 in lung epithelial cells. Cancer Immunol Res Feb 2021; 9 (2 Supplement):PO033. doi: 10.1158/2326-6074.TUMIMM20-PO033. 

2. Ganapathy V, Manyanga J, McGuire D, Brobst D, Wagener TL, Rubenstein D, Ramachandran I, Queimado L. Electronic cigarette aerosols increase cellular reactive oxygen species and induce significant oxidative DNA damage. Cancer Res July 1 2017; (77) (13 Supplement): 502. doi: 10.1158/1538-7445.AM2017-502.

Selected Conference Presentations:

1. Brobst D, Ganapathy V, Chissoe G, Sadhasivam B, Chinnaiyan M, Johnson K, and Queimado L. Oral Molecular Changes in Exclusive and Dual Users of Combustible and Electronic Cigarettes. 9th Annual Otolaryngology Head and Neck Surgery Research Day, April 2, 2022, OUHSC, Oklahoma City, OK, Oral Presentation.

2. Chinnaiyan M, Chissoe G, Acharya P, Sadhasivam B, Ganapathy V, Brobst D, Johnson K, Zhao YD, and Queimado L. Marijuana Use And Co-Use Among Tobacco And Non-Tobacco Products Users. 9th Annual Otolaryngology Head and Neck Surgery Research Day, April 2, 2022, OUHSC, Oklahoma City, OK, Oral Presentation.

3. Coles T, Sadhasivam B, Chinnaiyan M, Chissoe G, Ganapathy V, Brobst D, Johnson K, and Queimado L. Evaluation of E-cigarette Mutagenicity in Vapers Using Ames Test. 9th Annual Otolaryngology Head and Neck Surgery Research Day, April 2, 2022, OUHSC, Oklahoma City, OK. Oral Presentation. Won Jesus E. Medina M.D. Award for Excellence in Research by a Medical Student.

4. Ganapathy V, Brobst D, Manyanga J, and Queimado L. E-Cigarette Aerosol Increases Oxidative Stress And Alters Inflammatory Markers In Oral Cells. 9th Annual Otolaryngology Head and Neck Surgery Research Day, April 2, 2022, OUHSC, Oklahoma City, OK, Oral Presentation.

5. Milton A, Chinnaiyan M, Chissoe G, Brobst D, Sadhasivam B, Ganapathy V, and Queimado L. Puff Topography Among Single and Dual Users of Combustible Cigarettes, Pod-Based, and Mod-Based Electronic Cigarettes. 9th Annual Otolaryngology Head and Neck Surgery Research Day, April 2, 2022, OUHSC, Oklahoma City, OK. Oral Presentation.

6. Chinnaiyan M, Chissoe G, Acharya P, Sadhasivam B, Ganapathy V, Brobst D, Johnson K, Zhao YD, and Queimado L. Prevalence of marijuana use and co-use among combustible tobacco and electronic cigarette users. 2022 Stephenson Cancer Center Annual Cancer Research Symposium, March 4, 2022, Virtual Meeting. Virtual Poster.

7. Ganapathy V, Brobst D, Manyanga J, and Queimado L. E-Cigarette Aerosol Alters the Expression of Antioxidant and Detoxification Enzymes and Inflammatory Markers in Lung Cells. 2022 Stephenson Cancer Center Annual Cancer Research Symposium, March 4, 2022, Virtual Meeting. Virtual Pre-Symposium Poster.

8. Milton A, Chinnaiyan M, Chissoe G, Brobst D, Sadhasivam B, Ganapathy V, and Queimado L. Puffing Topography Among Exclusive Combustible Cigarette Users and Dual Users of Cigarettes and Pod-Based or Mod-Based Electronic Cigarettes. 2022 Stephenson Cancer Center Annual Cancer Research Symposium, March 4, 2022, Virtual Meeting. Virtual Poster.

9. Chinnaiyan M, Brobst D, Sadhasivam B, Ganapathy V, Johnston SE, Zhao YD, Krempl G, and Queimado L. DNA damage on HPRT as a biomarker of susceptibility to tobacco-induced cancer. American Association for Cancer Research (AACR) Annual Meeting 2021, April 10-15, 2021, Virtual Meeting. Virtual Poster. Awarded the Stephenson Cancer Center Trainee Research Conference and Workshop​ Travel Award.

10. Manyanga J, Ganapathy V, Brobst D, Wren J, and Queimado L. Exposure to E-Cigarette Aerosol Extracts Alters the Expression of Cytochrome P450 Mono-Oxygenase Enzymes. Society for Research on Nicotine and Tobacco (SRNT) 27th Annual Meeting, February 24-27, 2021, Virtual Meeting. Oral Pre-Recorded Virtual Online Podium Presentation.

11. Ganapathy V, Manyanga J, Brobst D, Georgescu C, Wren J, and Queimado L. E-Cig Aerosol Reduces the Expression of Key Antioxidant Regulators and Increases Inflammatory Markers. Society for Research on Nicotine and Tobacco (SRNT) 27th Annual Meeting, February 24-27, 2021, Virtual Meeting. Virtual Poster.

12. Ganapathy V, Manyanga J, Brobst D, Georgescu C, Wren J, and Queimado L. E-Cigarette Aerosol Alters Antioxidant Master Regulator and Modulates Inflammatory Responses in Oral Epithelial Cells. 2021 Stephenson Cancer Center Annual Cancer Research Symposium, February 5, 2021, Virtual Meeting. Virtual Poster.

13. Brobst D, Manyanga J, Georgescu C, Wren J, and Queimado L. Exposure to E-cigarette Aerosol Extracts Decreases the Expression of Key Proteins of the Innate Immune Response in Lung Epithelial Cells. 2021 Stephenson Cancer Center Annual Cancer Research Symposium, February 5, 2021, Virtual Meeting. Virtual Poster.

14. Chinnaiyan M, Brobst D, Sadhasivam B, Ganapathy V, Johnston SE, Zhao YD, Krempl G, and Queimado L. Quantification of DNA Damage on HPRT as a Biomarker of Cancer Risk. 2020 Stephenson Cancer Center Annual Cancer Research Symposium, February 7, 2020, OUHSC, Oklahoma City, OK. Poster.

15. Brobst D, Ganapathy V, Sadhasivam B, Chinnaiyan M, and Queimado L. Expanding and Validating PADDA: Moving a Novel DNA Damage Detection Assay to New Genes. 6th Annual Otolaryngology Head and Neck Surgery Research Day, April 27, 2019, OUHSC, Oklahoma City, OK. Oral Presentation.

16. Chinnaiyan M, Brobst D, Ganapathy V, Sadhasivam B, and Queimado L. Quantification of DNA damage on HPRT as a biomarker of cancer risk. 6th Annual Otolaryngology Head and Neck Surgery Research Day, April 27, 2019, OUHSC, Oklahoma City, OK. Oral Presentation.

17. Krutz M, Chinnaiyan M, Brobst D, Johnston SE, Zhao YD, and Queimado L. Correlations between DNA Damage at Diagnosis and Clinical Parameters in Patients with Head and Neck Squamous Cell Carcinoma. 6th Annual Otolaryngology Head and Neck Surgery Research Day, April 27, 2019, OUHSC, Oklahoma City, OK. Oral Presentation.

18. Brobst D and Queimado L. E-cigarette induced DNA strand breaks in oral cancer cells. 5th Annual Otolaryngology Head and Neck Surgery Research Day, April 7, 2018, OUHSC, Oklahoma City, OK. Oral Presentation.