Jordan P. Metcalf M.D.
Professor of Medicine
Pulmonary, Critical Care & Sleep Medicine Section
Department of Medicine
University of Oklahoma Health Sciences Center
800 N. Research Parkway, Rm 427
Oklahoma City, OK 73104
(405) 271-1966
Jordan-Metcalf@ouhsc.edu
Research Summary
The principal focus of my research laboratory is to understand the innate immune responses to infectious agents, including adenovirus, influenza virus, and bacterial pathogens such as Bacillus anthracis, at cellular and molecular levels. Beginning in 2002, we developed a human lung organ culture model in order to determine how pathogens stimulate, and sometimes evade, the immune response, and also how the human host attempts to respond to the pathogen. We use primary lung cells and organ culture models to explore host-pathogen interactions with regards to adenovirus, influenza, and B. anthracis and the effect of cigarette smoke exposure on host immune responses.
Currently my laboratory is working on the following research projects:
Determining mechanisms whereby cigarette smoke impairs antiviral innate responses and increases morbidity and mortality during influenza infections
Determining mechanisms of escape for the Bacillus anthracis from the human lung alveolar space during inhalational anthrax.
Determining mechanisms of enhanced pathogenesis of SARS-CoV-2 through alterations in the antiviral innate immune responses in the lung.
Laboratory Team
- Wenxin Wu, Ph.D., Research Assistant Professor
- Lee Booth M.S. Research Assistant
- Wei Zhang , Research Assistant
- Lili Tian, M.S., Research Fellow
- Jeremy Alexander, Research Technician
Current positions
Please contact for current openings in our laboratory.
Education:
M.D., University of Nebraska College of Medicine (AOA), 1984
Training:
Resident, Internal Medicine, University of Nebraska Medical Center, 1984-1988
Pulmonary and Critical Care Fellow, University of Nebraska Medical Center, 1988-1991
Research Fellow, University of Iowa, 1991-1993
Professional Experience:
Associate (Instructor), Department of Medicine, Section of Pulmonary and Critical Care Medicine, University of Iowa, 1993-1994
Assistant Professor, Department of Medicine, Section of Pulmonary, Critical Care & Sleep Medicine, University of Oklahoma Health Sciences Center, 1994-2000
Associate Professor, Department of Medicine, Section of Pulmonary, Critical Care & Sleep Medicine, University of Oklahoma Health Sciences Center, 2000-2007
Professor, Department of Medicine, Section of Pulmonary, Critical Care & Sleep Medicine, University of Oklahoma Health Sciences Center, 2007-present
Research Director, Department of Medicine, Section of Pulmonary, Critical Care & Sleep Medicine, University of Oklahoma Health Sciences Center, 2014-present
Select Publications (of 57 total):
Patel VI, Booth JL, Dozmorov M, Brown BR, Metcalf JP. Anthrax Edema and Lethal Toxins Differentially Target Human Lung and Blood Phagocytes. Toxins (Basel). 2020 Jul 20;12(7):E464. PMID: 32698436 PMCID: PMC7405021
Wu W, Zhang W, Tian L, Brown BR, Walters MS, Metcalf JP. IRF7 Is Required for the Second Phase Interferon Induction During Influenza Virus Infection in Human Lung Epithelia. Viruses. 2020 Mar 29;12(4):377. PMID: 32235406 PMCID: PMC7232147
Beigel JH, Aga E, Elie-Turenne MC, Cho J, Tebas P, Clark CL, Metcalf JP, Ozment C, Raviprakash K, Beeler J, Holley HP Jr, Warner S, Chorley C, Lane HC, Hughes MD, Davey RT Jr; IRC005 Study Team. Anti-influenza immune plasma for the treatment of patients with severe influenza A: a randomised, double-blind, phase 3 trial. Lancet Respir Med. 2019 Nov;7(11):941-950. PMID: 31582360 PMCID: PMC6941345
Wu W, Wang X, Zhang W, Tian L, Booth JL, Duggan ES, More S, Liu L, Dozmorov M,Metcalf JP. RIG-I Signaling via MAVS is Dispensable for Survival in Lethal Influenza Infection In Vivo. Mediators of Inflammation 018 Nov 8; 2018:6808934. PMID: 30532653; PMCID: PMC6250004.
More S, Yang X, Zhu Z, Bamunuarachchi G, Guo Y, Huang C, Bailey K, Metcalf JP, Liu L. Regulation of influenza virus replication by Wnt/β-catenin signaling. PLOS One 2018, 13. PMID: 29324866 PMCID: PMC5764324
Wang X, Wu W, Zhang W, Booth JL, Duggan ES, Tian L, More S, Zhao YD, Sawh RN, Liu L, Zou MH, Metcalf JP. RIG-I Overexpression Decreases Mortality of Cigarette Smoke Exposed Mice During Influenza A Virus Infection. Respir. Res. 2017, 18:166. PMID: 28865477, PMCID: PMC5581920.
Patel VI, Booth JL, Duggan ES, Cate S, White VL, Hutchings D, Kovats S, Burian DM, Dozmorov M,Metcalf JP. Transcriptional Classification and Functional Characterization of Human Airway Macrophage and Dendritic Cell Subsets. J Immunol. 2017, 198:1183-1201. PMID: 28031342 PMCID: PMC5262539
Wu W, Zhang W, Booth JL, Hutchings DC, Wang X, White V; Youness V; Cross CD; Zou MH; Burian D; Metcalf JP, Human Primary Airway Epithelial Cells Isolated from Active Smokers Have Epigenetically Impaired Antiviral Responses. Respir Res. 2016, 17:111. PMID: 27604339; PMCID: PMC5013564
Booth JL, Duggan ES, Patel VI, Langer M, Wu W, Braun A, Coggeshall KM, Metcalf JP, Bacillus anthracis spore movement does not require a carrier cell and is not affected by lethal toxin in human lung models, Microb Infect. 2016, 18:615-626. PMID: 27320392 PMCID: PMC5534360
Wu, W, Zhang W, Duggan ES, Booth JL, Zou MH, Metcalf JP. RIG-I and TLR3 Are Both Required for Maximum Interferon Induction by Influenza Virus in Human Lung Alveolar Epithelial Cells. Virology. 2015, 482:181-8.PMID: 25880109 PMCID: PMC4461467
Wu W, Zhang W, More S, Booth JL, Duggan ES, Liu L, Zhao YD, Metcalf JP.Cigarette smoke attenuates the RIG-I-initiated innate antiviral response to influenza infection in two murine models.Am J Physiol Lung Cell Mol Physiol. 2014, 307:L848-58. PMID: 25260755 PMCID: PMC4254961
Langer M, Duggan ES, Booth JL, Patel VI, Zander RA, Silasi-Mansat R, Ramani V, Veres TZ, Prenzler F, Sewald K, Williams DM, Coggeshall KM, Awasthi S, Lupu F, Burian D, Ballard JD, Braun A, Metcalf JP. Bacillus anthracis lethal toxin reduces human alveolar epithelial barrier function. Infect Immun. 2012, 80:4374-87. PMID: 23027535 PMCID: PMC3497415
Wu W, Zhang W, Booth JL, Metcalf JP. Influenza A(H1N1)pdm09 Virus Suppresses RIG-I Initiated Innate Antiviral Responses in the Human Lung. PLoS One. 2012;7:e49856. PMID: 23185463 PMCID: PMC3503992
Wu S, Metcalf JP, Wu W. Innate response to influenza virus. Curr Opin Infect Dis. 2011; 24:235-40. PMID: 21330918
Wu W, Patel KB, Booth JL, Zhang W, Metcalf JP. Cigarette smoke extract suppresses the RIG-I initiated immune response to influenza infection in human lung. Am J Physiol Lung Cell Mol Physiol. Am J Physiol Lung Cell Mol Physiol. 2011; 300:L821-30. PMID: 21335520 PMCID: PMC3119130
Wu W, Booth JL, Duggan ES, Wu S, Patel KB, Coggeshall KM, Metcalf JP. Innate immune response to H3N2 and H1N1 influenza virus infection in a human lung organ culture model. Virology. 2010, 396:178-88. PMID: 19913271 PMCID: PMC2789846
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel K, Ballard JD, Coggeshall KM, Metcalf JP. Resistance of Human Alveolar Macrophages to Bacillus anthracis Lethal Toxin. J Immunol, 2009, 183:5799-806. PMID: 19812208 PMCID: PMC2770336
Chakrabarty K, Wu W, Booth JL, Duggan ES, Coggeshall KM, Metcalf JP.Human Lung Innate Immune Response To Bacillus anthracis Spore Infection. Infect Immun. 75:3729-38, 2007. PMID: 17517878 PMCID: PMC1952005
Chakrabarty K, Wu W, Booth JL, Duggan ES, Coggeshall KM, Metcalf JP, Bacillus Anthracis Spores Stimulate Cytokine And Chemokine Innate Immune Responses In Human Alveolar Macrophages Through Multiple MAPK Pathways. Infect Immun. 74:4430-8, 2006. PMID: 16861629 PMCID: PMC1539616
Wu W., Booth J.L., Coggeshall KM, Metcalf JP, Calcium-Dependent Viral Internalization is Required for Adenovirus Type 7 Induction of IL-8 Protein. Virology.355:18-29, 2006. PMID: 16890267
Booth, JL, KM Coggeshall, BE Gordon, Metcalf JP.Adenovirus 7 Induces Interleukin-8 In A Lung Slice Model And Requires Activation Of Erk. J Virol. 78:4156-64, 2004. PMID: 15047831 PMCID: PMC374302
Sanchez TA, Booth JL, Metcalf JP. TNF Activation by Adenovirus E1A 13S CR3 Occurs in a Cell Dependent and Cell Independent Manner. Am J Physiol Lung Cell Mol Physiol 283: L619-27, 2002.
Alcorn MJ, Booth JL, Coggeshall KM, Metcalf JP. Adenovirus Type 7 Induces Interleukin-8 Production via Activation of Extracellular Regulated Kinase 1/2. J Virol. 75:6450-9, 2001. PMID: 11413312 PMCID: PMC114368
Sanchez TA, Booth JL, Habib I, Metcalf JP.The Zinc Finger And Carboxyl Regions Of Adenovirus E1a 13s CR3 Are Important For Transactivation Of The Cytomegalovirus Major Immediate Early Promoter By Adenovirus. Am J Resp Cell Mol Biol 23:670-7, 2000. PMID: 11062146
Booth JL, Metcalf JP, Type-Specific Induction of Interleukin-8 by Adenovirus. Am J Resp Cell Mol Biol 21:521-527, 1999. PMID: 10502562
Metcalf JP, Monick MM, Hunninghake GW.The adenoviral E1A gene product upregulates the cytomegalovirus major immediate early gene promoter in inflammatory cells.Am J Resp Cell Mol Biol 10:448-52, 1994. PMID: 8928812
Metcalf JP, Armitage JO, Rennard SI, et. al.Corticosteroids as adjunctive therapy for diffuse alveolar hemorrhage associated with bone marrow transplantation.Am J Med 96:327-34, 1994. PMID: 8166151
Metcalf JP, Thompson AB, Gossman GL, et al.Gcglobulin functions as a cochemotaxin in the lower respiratory tract.A potential mechanism for lung neutrophil recruitment in cigarette smokers. Am Rev Resp Dis 143:844-9, 1991. PMID: 2008995
Metcalf JP, Casey CA, Sorrell MF, Tuma DJ.Chronic ethanol administration alters hepatic surface membranes as evidenced by decreased concanavilin A binding.Proc Sci Exp Biol Med 185: 1-5, 1987. PMID: 3575324
Blake CA, Metcalf JP, Hendricks SE.Anterior pituitary gland secretion after forebrain ablation:periovulatory gonadotropin release.Endocrinology 111: 789-93, 1982. PMID: 6809447