Super-Resolution Imaging Core (SRIC)

The microscope is built on a Nikon Ti2 inverted fluorescence microscope equipped for Differential Interference Contrast and fluorescence imaging in the blue, green, red, and far red channels at magnifications of 4X, 10X, 20X, and 100X.

• Super-resolution imaging modalities: TIRF, Structured Illumination Microscopy (SIM), and Stochastic Optical Reconstruction Microscopy (STORM)
• Stage top incubator for live cell imaging
• Nikon Elements software for image acquisition and analysis
• User fee: $20/hr

The microscope is built on a Nikon Ti2 inverted fluorescence microscope equipped for Differential Interference Contrast and fluorescence imaging in the blue, green, red, and far red channels at magnifications of 4X, 10X, 20X, 40X, 60X, and 100X.

• SoRa attachment enables super-resolution imaging
• Spinning disk design permits confocal imaging through specimens up to 200-300 micrometers thick
• Stage top incubator for live cell imaging
• Fast galvo scanner for dynamic imaging methods including FRAP, photoconversion, and photostimulation
• User fee: $20/hr

This multi-functional microscope is built on a Nikon Ti2 inverted fluorescence microscope equipped with two independent illumination decks for high-speed super-resolution and widefield imaging.

• First deck configured for high-speed super-resolution imaging using Nikon AX-R/NSPARC confocal imaging
• Supports Fluorescence Lifetime Imaging (FLIM) via PicoQuant software and hardware for Time-Correlated Single Photon Counting
• Enables local environment sensing and detection of molecular interactions via FLIM/FRET
• Stage-mounted live-cell imaging chambers for confocal, FLIM, or widefield imaging modes
• Nikon Elements software for image acquisition and PicoQuant software for FLIM analyses

This system combines gentle light sheet imaging and fast image acquisitions to enable dynamic imaging of full sample volumes at subcellular resolutions. The lattice-structured light sheet greatly reduces photobleaching and phototoxicity, providing unsurpassed imaging of living specimens for hours to days. This system includes a Lattice LightSheet microscope utilizing Bessel beam lattice sheet illumination via cylindrical lenses and high-speed SLM for multicolor imaging, annular mask array for various lightsheets, galvo mirrors to control lattice movement in x and z, cameras in image space to inspect the lattice pattern, 25x/1.1NA water immersion detection objective, 28x/0.71NA water immersion illumination objective, x,y translation stages and piezo imaging objective control.