Education:
| 2012 |
PhD |
Medical Science, Kyushu University, Fukuoka, Japan |
| 2007 |
MD |
Medicine, Akita University, Akita, Japan |
Clinical/Research Interests:
The Kanie lab is interested in elucidating the molecular mechanisms of the formation of the primary cilium.
The primary cilium is an organelle that extends from the cell surface whose central function is to serve as a signaling hub, sensing numerous extracellular signals via receptors (e.g., GPCRs) that are specifically accumulated on the ciliary portion of the cell membrane. This organelle is fundamentally important for vertebrate development. It modulates survival and patterning of embryos via critical signaling pathways, such as Hedgehog signal transduction. Structural and/or functional defects in primary cilia result in developmental disorders and syndromes ranging from neural tube, brain and skeletal malformations, to obesity, intellectual disability, retinal degeneration, and polycystic kidney disease, which all together are called ciliopathies.
The Kanie lab strives to understand how this tiny organelle forms, using various state-of-the art techniques, including super resolution microscopies (STORM, 3D-SIM, and optical reassignment based super resolution live cell imaging), mass spectrometry, and biochemistry. The Kanie lab also strives to generate excellent next generation scientists. The lab is committed to provide sufficient education to perform accurate and reproducible research, conduct well-planned experiments with optimal controls, develop a broad range of knowledge, and discuss a wide scope of biological topics with joy.
Select Publications:
- Kanie T, Liu B, Love JF, Fisher SD, Gustavsson A-K, Jackson PK. A hierarchical pathway for assembly of the distal appendages that organize primary cilia. Elife. 2025 Jan 30;14:e85999. doi: 10.7554/eLife.85999. PMID: 39882846
- Kanie T, Ng R, Abbott KL, Pongs O, Tanvir NM, Lorentzen E, Jackson PK. Myristoylated Neuronal Calcium Sensor-1 captures the ciliary vesicle at distal appendages. Elife. 2025 Jan 30;14:e85998. doi: 10.7554/eLife.85998. PMID: 39882855
- Kanie T, Abbott KL, Mooney NA, Plowey ED, Demeter J, and Jackson PK. The CEP19-RABL2 GTPase complex binds IFT-B to initiate intraflagellar transport at the ciliary base. Developmental Cell, 2017 Jul 10;42(1):22-36.e12. doi: 10.1016/j.devcel.2017.05.016. PMID: 28625565